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spp1  (R&D Systems)


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    R&D Systems spp1
    Identification of CD59 as a human-specific biomarker for the proximal nephron. ( A ). Schematic of the screening approach: normal human urinary proteome, in vitro cultured kidney organoid transcriptome, and transcriptome of engrafted kidney organoids were cross-referenced for common proteins/transcripts. ( B ). Amino acid identities of human versus mouse paralogs and results of measurement of human versus mouse using commercial ELISA kits. ( C ). Expression of <t>SPP1</t> in adult human kidney. ( D ). Expression of CD59 in adult human kidney. ( E ). Results of ELISA measurement of 3 male and 3 female urine samples from humans and mice. #NUM! indicates a measurement below the threshold of detection, 3.21 × 10 4 is the Glomax plate reader convention for 3.21 × 10 4 . ( F ). Schematic showing the origin of CD59 secretion (green) and uromodulin (THP secretion (purple).
    Spp1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 143 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/spp1/product/R&D Systems
    Average 95 stars, based on 143 article reviews
    spp1 - by Bioz Stars, 2026-03
    95/100 stars

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    1) Product Images from "In Vivo Assessment of Laboratory-Grown Kidney Tissue Grafts"

    Article Title: In Vivo Assessment of Laboratory-Grown Kidney Tissue Grafts

    Journal: Bioengineering

    doi: 10.3390/bioengineering10111261

    Identification of CD59 as a human-specific biomarker for the proximal nephron. ( A ). Schematic of the screening approach: normal human urinary proteome, in vitro cultured kidney organoid transcriptome, and transcriptome of engrafted kidney organoids were cross-referenced for common proteins/transcripts. ( B ). Amino acid identities of human versus mouse paralogs and results of measurement of human versus mouse using commercial ELISA kits. ( C ). Expression of SPP1 in adult human kidney. ( D ). Expression of CD59 in adult human kidney. ( E ). Results of ELISA measurement of 3 male and 3 female urine samples from humans and mice. #NUM! indicates a measurement below the threshold of detection, 3.21 × 10 4 is the Glomax plate reader convention for 3.21 × 10 4 . ( F ). Schematic showing the origin of CD59 secretion (green) and uromodulin (THP secretion (purple).
    Figure Legend Snippet: Identification of CD59 as a human-specific biomarker for the proximal nephron. ( A ). Schematic of the screening approach: normal human urinary proteome, in vitro cultured kidney organoid transcriptome, and transcriptome of engrafted kidney organoids were cross-referenced for common proteins/transcripts. ( B ). Amino acid identities of human versus mouse paralogs and results of measurement of human versus mouse using commercial ELISA kits. ( C ). Expression of SPP1 in adult human kidney. ( D ). Expression of CD59 in adult human kidney. ( E ). Results of ELISA measurement of 3 male and 3 female urine samples from humans and mice. #NUM! indicates a measurement below the threshold of detection, 3.21 × 10 4 is the Glomax plate reader convention for 3.21 × 10 4 . ( F ). Schematic showing the origin of CD59 secretion (green) and uromodulin (THP secretion (purple).

    Techniques Used: Biomarker Discovery, In Vitro, Cell Culture, Enzyme-linked Immunosorbent Assay, Expressing



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    Identification of CD59 as a human-specific biomarker for the proximal nephron. ( A ). Schematic of the screening approach: normal human urinary proteome, in vitro cultured kidney organoid transcriptome, and transcriptome of engrafted kidney organoids were cross-referenced for common proteins/transcripts. ( B ). Amino acid identities of human versus mouse paralogs and results of measurement of human versus mouse using commercial ELISA kits. ( C ). Expression of <t>SPP1</t> in adult human kidney. ( D ). Expression of CD59 in adult human kidney. ( E ). Results of ELISA measurement of 3 male and 3 female urine samples from humans and mice. #NUM! indicates a measurement below the threshold of detection, 3.21 × 10 4 is the Glomax plate reader convention for 3.21 × 10 4 . ( F ). Schematic showing the origin of CD59 secretion (green) and uromodulin (THP secretion (purple).
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    Identification of CD59 as a human-specific biomarker for the proximal nephron. ( A ). Schematic of the screening approach: normal human urinary proteome, in vitro cultured kidney organoid transcriptome, and transcriptome of engrafted kidney organoids were cross-referenced for common proteins/transcripts. ( B ). Amino acid identities of human versus mouse paralogs and results of measurement of human versus mouse using commercial ELISA kits. ( C ). Expression of <t>SPP1</t> in adult human kidney. ( D ). Expression of CD59 in adult human kidney. ( E ). Results of ELISA measurement of 3 male and 3 female urine samples from humans and mice. #NUM! indicates a measurement below the threshold of detection, 3.21 × 10 4 is the Glomax plate reader convention for 3.21 × 10 4 . ( F ). Schematic showing the origin of CD59 secretion (green) and uromodulin (THP secretion (purple).
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    Identification of CD59 as a human-specific biomarker for the proximal nephron. ( A ). Schematic of the screening approach: normal human urinary proteome, in vitro cultured kidney organoid transcriptome, and transcriptome of engrafted kidney organoids were cross-referenced for common proteins/transcripts. ( B ). Amino acid identities of human versus mouse paralogs and results of measurement of human versus mouse using commercial ELISA kits. ( C ). Expression of SPP1 in adult human kidney. ( D ). Expression of CD59 in adult human kidney. ( E ). Results of ELISA measurement of 3 male and 3 female urine samples from humans and mice. #NUM! indicates a measurement below the threshold of detection, 3.21 × 10 4 is the Glomax plate reader convention for 3.21 × 10 4 . ( F ). Schematic showing the origin of CD59 secretion (green) and uromodulin (THP secretion (purple).

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    Article Title: In Vivo Assessment of Laboratory-Grown Kidney Tissue Grafts

    doi: 10.3390/bioengineering10111261

    Figure Lengend Snippet: Identification of CD59 as a human-specific biomarker for the proximal nephron. ( A ). Schematic of the screening approach: normal human urinary proteome, in vitro cultured kidney organoid transcriptome, and transcriptome of engrafted kidney organoids were cross-referenced for common proteins/transcripts. ( B ). Amino acid identities of human versus mouse paralogs and results of measurement of human versus mouse using commercial ELISA kits. ( C ). Expression of SPP1 in adult human kidney. ( D ). Expression of CD59 in adult human kidney. ( E ). Results of ELISA measurement of 3 male and 3 female urine samples from humans and mice. #NUM! indicates a measurement below the threshold of detection, 3.21 × 10 4 is the Glomax plate reader convention for 3.21 × 10 4 . ( F ). Schematic showing the origin of CD59 secretion (green) and uromodulin (THP secretion (purple).

    Article Snippet: Assays for uromodulin (Human Uromodulin DuoSet ELISA DY5144-05, R&D Systems, Minneapolis, MN, USA), CD59 (Human CD59 ELISA ab263893, Abcam, Waltham, MA, USA), PSAP (Human PSAP/Prosaposin (Sandwich ELISA) ELISA LS-F35235, Lifespan Biosciences, Lynnwood, MA, USA), cubilin (Human CUBN/Cubilin (Sandwich ELISA) ELISA LS-F38077, Lifespan Biosciences, Lynnwood, MA, USA), SPP1 (Human Osteopontin (OPN) Quantikine ELISA DOST00, R&D Systems, Minneapolis, MN, USA), and HSPG2 (HSPG2 elisa kit: Human Basement membrane-specific heparan sulfate proteoglycan core protein ELISA MBS765938, MyBioSource, San Diego, CA, USA) were used according to the manufacturer’s instructions and assays were read on a Glomax Explorer (Promega, Madison, WI, USA) plate reader.

    Techniques: Biomarker Discovery, In Vitro, Cell Culture, Enzyme-linked Immunosorbent Assay, Expressing